culture: coolio25026
age: 13 DIV
temperature: 30C
ROI: 01

white LED level: 20
blue LED level: 75
exposure: 50ms

x-position: -0.52769
y-position: 1.45814
z-position: 27um

because electrode 24 had a nice big single unit, I positioned objective over electrodes 24, 25, 34, and 35


recording for 5min, with 10s before and after


imaging notes: coolio25026_ROI-01_no-drug_ChR2-stim_3
---------------------
num_frames: 5000 (target: >=5min,20sec), I stopped it at 4017 because that was enough (4000 should be fine in future)
total time: 332.4172 s
corresponding MCD file: coolio25026_ROI-01_no-drug_ChR2-stim_0003.mcd



imaging notes: coolio25026_ROI-01_no-drug_no-stim_2
---------------------
num_frames: 4100
total time:  339.454 s
corresponding MCD file: coolio25026_ROI-01_no-drug_ChR2-stim_0003.mcd

same stim protocol as first recording, but turned blue light off (but digital pulses will still be there)


****** ADDED CNQX at 3:16PM ******
did a 10min MEA-only recording
during which I did see some bursts
in fact it might be bursting more
some of them are elongated i think...

since there was a long of bursting, i gave the culture another 5min and 30sec (and recorded)
still bursting.
well, we'll just go ahead and start the imaging recordings... 15min should be plenty, and some cultures just burst in CNQX (oh well)


imaging notes: coolio25026_ROI-01_CNQX_no-stim_2
---------------------
num_frames: 4100
total time:  339.351 s
corresponding MCD file: coolio25026_ROI-01_CNQX_no-stim_0002.mcd

again, same stim protocol as first recording, but turned blue light off (but digital pulses will still be there)

lots of bursting throughout
i accidentally stopped the MEA recording prematurely (but still captured all the imaging part... stopped around 320s)


imaging notes: coolio25026_ROI-01_CNQX_ChR2-stim_2
---------------------
num_frames: 4100
total time:  339.52 s
corresponding MCD file: coolio25026_ROI-01_CNQX_ChR2-stim_0003.mcd

same stim protocol as first recording, now with blue light turned back on